2.Guidelines for calculating and reporting plate counts have been changed to conform with the anticipated changes in the 16th edition of Standard Methods for the Examination of Dairy Products ( 2) and the International Dairy Federation (IDF) procedures ( 6). For procedural details of the standard plate count, see ref.

The automated spiral plate count method for the examination of foods and cosmetics ( 5), outlined below, conforms to AOAC Official Methods of Analysis, sec. The suitable colony counting range ( 10) is 25-250. The conventional plate count method for examining frozen, chilled, precooked, or prepared foods, outlined below, conforms to AOAC Official Methods of Analysis, sec. Detailed procedures for determining the APC of foods have been developed by the Association of Official Analytical Chemists (AOAC) ( 3) and the American Public Health Association (APHA) ( 1). The aerobic plate count (APC) is intended to indicate the level of microorganism in a product. Peeler (ret)įor additional information, contact Guodong Zhang. It is envisioned that viscous SC IFC paves the way for IFC to be really usable in practice with clogging-free, accurate, and sensitive performance.Authors: Larry Maturin (ret.) and James T. Viscous SC IFC also shows satisfactory ability to distinguish different types of cancer cells and different subtypes of human breast cancer cells. MC, and demonstrated that viscous non-conductive PEG solution achieved an improved sensitivity (7.92×) and signal-to-noise ratio (1.42×) in impedance measurement, with the accuracy maintained and free of clogging. Through modeling analysis and experiments, we confirmed the accuracy (error < 1.60% ± 4.71%) of SC w.r.t. By placing MC and SC in series in the same microfluidic chip, we established an evaluation platform to prove the hypothesis. Herein, we hypothesize that the viscosity of the non-conductive liquid is the key to the performance of SC, and propose to employ non-conductive viscous sheath flow in SC to unlock the tradeoff between sensitivity and throughput, while ensuring measurement accuracy. Current sheath constriction (SC) solutions lack systematic evaluation of the performance and proper guidelines for the sheath fluid. As a label-free and high-throughput single cell analysis platform, impedance flow cytometry (IFC) suffers from clogging caused by a narrow microchannel as mechanical constriction (MC).

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